DNA CONTENT/CELL CYCLE ANALYSIS
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Fresh, frozen, or formalin fixed, paraffin embedded tumor tissue; peripheral blood or cell suspensions; miscellaneous body fluids.
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0.5 g tissue on dry ice, frozen; fresh tissue in tissue culture media on ice; fresh fluids on wet ice; peripheral blood (lavender, EDTA) at ambient temperature; cell suspensions fixed in 50% methanol on ice, or paraffin embedded tissue block at ambient temperature. Use refrigerant pack in place of wet ice, if transport time is > 1 hour.
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Peripheral blood, fresh fluids on ice and fresh tissue in tissue culture media - 24 hours;
Fixed cell suspensions or tissues - indefinite; frozen tissue - See remarks below.
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No tumor tissue remaining on block. Samples fixed in Bouin's or B-5.
Frozen samples that have thawed.
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CRITICAL FROZEN. Tissue should be frozen within 30 minutes of collection. Sample should be transported from surgery or biopsy site on on wet ice and handled immediately by a pathologist. Pathologist should confirm that the sample is tumor tissue and send a sample as free as possible from necrotic and non-tumor tissue. Sample must not thaw in transit. Pack accordingly. Tissue may be snap frozen in liquid nitrogen or frozen in a cryostat, then placed in the container and held in the freezer or on dry ice. Alternately, use a biopsy tissue cassette for freezing, storage and shipment. Tissues may be stored for short periods of time at -20 degrees C if no ultra-low temperature storage is available.
Paraffin embedded tissue or tissue fixed in well-buffered formalin are also acceptable.
Please provide clinical information and surgical pathology report. If no normal tissue is included in the block, please supply control tissue block processed in parallel.
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SEE REPORT. Report components include: Tumor verification of stained slide, graph of DNA content, cell cycle analysis of DNA diploid and DNA aneuploid populations and pathologist's interpretation of ploidy and S-phase data.
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| ICD-9 (Dx) CODE INFORMATION |
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