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DNA CONTENT/CELL CYCLE ANALYSIS, MISCELLANEOUS
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| Tissue, fluids, peripheral blood or cell suspensions.
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| 0.5 g tissue on dry ice, frozen; fresh tissue on saline-soaked gauze or in tissue culture media on ice; fresh fluids on wet ice; peripheral blood (lavender, EDTA) at ambient temperature; cell suspensions fixed in 50% methanol on ice, or paraffin embedded tissue block at ambient temperature. Use refrigerant pack in place of wet ice, if transport time is < 1 hour.
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| CRITICAL FROZEN. Tissue should be frozen within 30 minutes of collection. Sample should be transported from surgery or biopsy site on wet ice and handled immediately by a pathologist. Pathologist should confirm that the sample is tumor tissue and send a sample as free as possible from necrotic and non-tumor tissue. Sample must not thaw in transit. Pack accordingly. Red tissue containers are provided to help insure that these critical samples are not mishandled at any step. Tissue may be snap frozen in liquid nitrogen or frozen in a cryostat, then placed in the container and held in the freezer or on dry ice. Alternately, use a red biopsy tissue cassette for freezing, storage and shipment. Tissues may be stored for short periods of time at -20 degrees C if no ultra-low temperature storage is available. When ordering supplies from Client Services, please specify "tissue container" or "biopsy cassette". Paraffin embedded tissue or tissue fixed in well-buffered formalin are also acceptable. Please provide clinical information.
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| Report components include: Tumor Verification, DNA Index, DNA Content, % Aneuploid, % S-phase, S-phase interpretation, copy of histogram.
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| ICD-9 (Dx) CODE INFORMATION |
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